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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been noticed in many tumors, which ends up in chromosome instability, and thus may perhaps add to tumorigenesis. This protein was located to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a critical part in advertising cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two additional splice variants were being described but specific nucleotide sequence information and facts isn't yet readily available.
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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide commonly contains the epitope identified because of the antibody.
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This manual outlines even further achievable optimization of mobile and immunoassay parameters to make certain the very best success are...
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This tutorial outlines more possible optimization of cellular and immunoassay parameters to make certain the very best benefits are...
Antibodies bound to the blocking peptide not bind to the epitope about the target protein. By comparing the staining through the blocked antibody vs . the antibody on your own, you can see which staining is certain.
Focus on Information and facts Cyclin E belongs on the highly conserved cyclin household, whose users are characterised by a spectacular periodicity in protein abundance through the cell cycle. Cyclins operate as regulators of CDK kinases. Unique cyclins exhibit distinctive expression and degradation patterns which add into the temporal coordination of each and every mitotic occasion. Cyclin E types a fancy with and functions like a regulatory subunit of CDK2, read more whose action is necessary for mobile cycle G1/S changeover. This protein accumulates in the G1-S phase boundary and is degraded as cells progress by S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by each antibodies...
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